Biochemistry is the study of the molecules of life-proteins, lipids, carbohydrates and nucleic acids. Studying the structures and properties of these molecules leads to a better understanding of life as a whole. Laboratory Manual in Biochemistry tries to address and compile some of the routinely used protocols in biochemistry. It aims not only to bring together the protocols, but also to help students understand the principles behind the procedures. The objective is to give students a general view of biochemistry. Even though the field of biochemistry is vast and exhaustive, an effort has been made to list some of the protocols that could serve as a foundation for starting any biochemical investigation.